A technique for ?xating specimens of tissue, involving a minimum of chemical and physical alteration. The histological specimen is immersed in a chemical, isopentane, which has been cooled in liquid air to a temperature just below 200 °C. This preserves the tissue instantly without large ice crystals forming – these would result in structural damage. The specimen is then dehydrated in a vacuum for three days, after which it can be examined using a MICROSCOPE.